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Jigen obviously wasn't hiding his chakra when he came to the leaf.His chakra was described as insanely sinister when he first entered the village to the point where Ino had trouble standing.
Now that I think of it, negative emotion sensing should be tied to chakra sensing.
So nullifying chakra signature entirely should also hide all forms of emotions. After all, chakra is directly from one's soul and body, and sums up the entire energy within a person.
Karin could detect emotional fluctuations by simply reading one's chakra.
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