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Why would that effect them? If they are completely capable of surviving normally with no soul then destroying one forced into them shouldn't have an effect.
Actually, the OP gave an example of someone conceptually binding someone a soul, not that "This being now requires a soul". Even if it's conceptual, this being never needed a soul in the first place so binding them one and killing it wouldn't do anything.
Id say unless the target has good enough resistance to their conceptual hax, then it should work on them. Even then itd probably still just be immunity negation.
Again, conceptually binding a naturally soulless being a soul and then killing it wouldn't do anything. It'd essentially be attaching a spare part to the being and then taking it away. You'd need to make it so the being REQUIRES a soul for killing their new soul to work.