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Regen neg comes from haki
Busushoku negates elemental intangibility and regeneration of Logia fruits, as well as unnatural regeneration caused by other devil fruits like the Tori-Tori No Mi Model Phoenix and possibly the Bara Bara No Mi

Ryuko's regeneration isn't caused by a devil fruit. It's her body's biological, natural regeneration. So Zoro's regen negation won't do anything
 
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