This is the last frame E-Soul is visible, which is around the crosswalk, and his lightning trail leads to the end of the road.
The car resembles a Ford F-250; its tire sizes range from 32 inch to 34 inch, lets assume 33 inch or 0.8382 m.
Tire: 67 px = 0.8382 m
Gap between lines: 91 px = (91 * 0.8382) / (67) = 1.13845074627 m
Gap between lines: 68 px = 1.13845074627 m
length of lines: 54 px = (54 * 1.13845074627) / (68) = 0.90406382792 m
line + gap = 1.13845074627 + 0.90406382792 = 2.04251457419 m
Counting the lines toward the next lightpole, there are twelve with gap for one more line, so around 13.
Distance between light poles: 13 * 2.04251457419 = 26.5526894645 m
There is a light pole by the crossroad where E-Soul dashed from. The number of light poles after the crossroad towards the end of the road is nine.
Distance E-Soul dashed: 9 * 26.5526894645 = 238.97420518 m
E-Soul disappeared within a single frame, so 1/24 seconds, or 0.04166666666 s
E-Soul's speed: 238.97420518 / 0.04166666666 = 5735.38092524 m/s [Mach 16.72]