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Let's start with the most straightforward definition. Ki is basically the life force an energy that exists within all living beings and which, with proper training, can be channeled and externalized and used in various types of techniques, from attacks to other uses, such as allowing its user to fly. As Ki is literally a person's life force, it is tied to their physiology: this is why Goku and other characters need to eat so much after using so much energy in fights. It's safe to say that proper rest and nutrition are enough to restore a person's Ki. That's even why the healing properties of the Seeds of the Gods can restore a fighter's energy levels. This also means that using your energy in a fight will cause you tremendous physical fatigue.
First evidence of ki being invisible
What did you fret over when thinking up techniques like the Kamehameha and their poses?
Techniques that are plain but effective aren’t suited for manga. Stuff like strangleholds and locking techniques are troublesome to draw and hard to make very flashy. Even the Kamehameha is a technique that expels “ki”, which by nature is invisible, but I was able to make it flashy by hitting upon the idea of portraying it as something that could be seen with the naked eye. With that pose too, I tried out a lot of different ones. What’s more, I had to think up special characteristics for each character’s techniques, but I ran out of patterns really fast, so it was tough (laughs).
Toriyamas column for "Dragon Ball Extreme Battle Collection: Round 02"
Second quote saying that ki is invisible
Dragon Ball seems to have influenced many video games and manga. Where did you get the idea for the attacks that appear in Dragon Ball, such as the kamehameha and the whole idea of chi power?
Chi [also spelled “Ki”–Editor] has been used in China since ancient times, but it’s supposed to be formless and invisible. However, in manga, in order to make it easier for any reader to grasp, it was necessary to give it a shape. For the kamehameha, I myself did a lot of different poses and chose the one that I thought would work the best.
The January 2003 of Shonen Jump
In episode 4 of Dragon Ball Daima ends up explaining again for the third time that ki is an invisible energy.
Basically, those visual and flashy effects are nothing less than to draw our attention to be able to see the ki, since the ki itself is invisible.
New ability that ki users will have on their ki manipulation page invisibility: